Diversity of cultivable actinomycetes in 6 species of herbivore feces

A large number of microbes existed in animal feces. To provide new sources for discovering new leader of drugs, the diversity of cultivable actinobacteria of animal feces have been studied. Fresh fecal samples of 6 species of herbivore feces were collected. The pure cultures of actinobacteria were isolated from these samples by using 5 media. The 16S rDNA gene sequences of 386 selected strains were analyzed, and the phylogenetic analysis was carried out. The study results shown that actinomycete diversity of each animal feces were different from each other; a total of 29 genera of the Class Actinobacteria were identified from the 6 species of animal feces; 15 genera were identified from fecal samples of Sika Deer(Cervus nippon)and Chinese Bamboo Rat(Rhizomys sinensis)respectively; and 1 new genus was discovered from Rhino(Rhinoceros sondaicus) feces. Members of three genera, Streptomyces, Rhodococcus and Microbacterium and Order Micrococcales were dominant groups in the 6 species of animal feces. This is a distinct feature of cultivable fecal actinomycete community differing from those in soil, plant, and marine environment. Selective isolation methods for fecal actinomycetes are described.